Sanger Sequencing Order Entry

Step 1: Create a batch

• Navigate to Batches -> New Batch

• Click on the link "New Order" in the table for the study for which a batch is to be created.

• Select the type of order to be placed with the help of 2 radio buttons for “ABI -3730 Platform” or “Illumina Genome Analyzer” platform.

For Ordering Services on "ABI-3730 Platform", follow the below steps

Step 2: Batch Definition

• Provide the batch details in the “Batch Definition” page. Provide the batch name, and Sample Type. Say, Batch Name is “Seq_Batch_1” and Sample Type “is “DNA” from the drop-down.

• DNA Plate Submission: Click 'Download Template' and save the Excel template to your computer.

• Fill in the details in file. Do not remove the first 2 lines of the file.

• Example sample excel file
  • To fill out this excel sheet with information regarding the samples that you will be submitting
    
  • ‘Sample Identifier’ must be unique. Sample identifier should only contain numbers, letters, dash, underscore, parentheses, period, and space
    
  • ‘Plate Label’ should be the same for all samples being submitted on the same plate. If samples are in tubes, each tube label must be unique.
    
  • Fill in the ‘Well’ column only if you are submitting a plate
    
  • If you wish to add annotation fields you can do so under the ‘Add additional annotation columns here’ heading.
    
  • All Other fields are optional but it is recommended that you take the time to fill out these fields now so that they are pre-filled during the following steps.
    
  • Return to the GIGPAD browser window and click ‘Upload’.

• To upload DNA sample file for new batch:
  • Click Upload..., a dialog should appear, navigate to the location where the excel sheet you just created is stored. Select the file.
  • Click ‘OK' to upload.
    
  • Once upload is done, the File Name and File Size should be displayed in the Sample Input Files box
    
  • Click on “Next Step (Sample Description)”.
    

Step 3: Select Services

Select the service to be performed on your samples from this drop down

• Provide the "Billing Information" and click on Next Step(Review Order) >> to proceed

Step 4: Billing Information

• This is an invoice payment option. If you would like to have an invoice sent to the selected recipient, simply enter in a percentage of the total bill you would like to allocate.
  • Select the PeopleSoft number you will be billing your services to.

Step 5: Review and Create Order

• Review your order. If you are satisfied with your order click 'Submit' otherwise the 'Previous' button to go back and edit your order. Or use 'Save and Finish Later' to return to your order at a later time (Your order will be saved in the browse window).
• Click on Submit Order>> for order submission.

Step 6: Order Confirmation

• Click on the link "here", to generate a printer-friendly version of the order confirmation.

• Print the order confirmation